Book Review - Cloning and Isolation of Taq DNA polymerase using pET expression system

The book titled 'Cloning and Isolation of Taq DNA polymerase using pET expression system - Molecular Cloning' was written by Sindhur Reddy Mandepudi and published by LAMBERT Academic Publishing of Germany. The book was reviewed in Eenadu daily by eminent scientist from IICT, Hyderabad. Here is the brief review of the book. The book is comprehensive that contains Four major parts, Part I gives the details of literature review, PCR Technique, Vectors and DNA modification enzymes. In Part II he could illustrates types of restriction enzymes and analytical techniques where as Part III has Materials and Methods finally results are described in Part IV. Author mainly collected literature from various sources and incorporated in first three chapters. In the last chapter the author has highlighted the results part which is the main essence of the book.

Thermostable enzyme for DNA amplification is required for PCR and such DNA polymerase from thermophilic bacteria Thermus aquaticus, Taq, is the most commonly used in molecular biology for production of large amount of Taq polymerase. The process used by the author to obtain Taq polymerase is quite economical and found to be high efficiency than the commercial Taq. The results are quite encouraged and can be used in molecular biology. The author is studying his B.Tech (Biotechnology) in Bharat Institute of Engineering and Technology, Hyderabad.

Though the major portion of the book contains literature and standard methodology collected from various sources the importance of economic production of large amount of Taq polymerase from clone bacteria is worth reading and notable. Overall the book is well written with suitable references in a simple form that can be absorbed by much student community belong to biotech sector. The schematic steps regarding cloning, isolation of Taq polymerase, chemical used and related steps in molecular biology are well described and I strongly recommend the book for B.Tech and other under graduate students of biotech. Author can concentrate on publishing the work in any reputed journal.

Related Posts