10th Biology Photosysthesis and Light - New Syllabus

Light and Photosynthesis

In Priestley’s time, scientists didn’t quite understand about energy, but later on much was discovered about it. If combining oxygen with carbon and hydrogen atoms to form carbon dioxide and water produced energy , what about the reverse? What about forming oxygen again and putting it back in the air. Eventually, scientists learned that the energy situation would also reverse. Oxygen formation would use up energy . This meant that if plants formed oxygen they had to get energy to make that possible. Where did the energy come from?

A Dutch scientist, Jan Ingenhousz ( 1730-1799), found the answer. He kept studying the way in which plants formed oxygen and, in 1779, noticed that this only happened in the light. In an experiment with the aquatic plant, Hydrilla, he observed that in bright sunlight, small bubbles formed around the green parts while in the dark they did not form. He also found that the gas present in the bubbles was oxygen.

It was further confirmed when Engelman in the early 20th century ingeniously detected the point of maximum photosynthesis. He used a strand of algae and exposed it to different colours of light (the colours that we see in a rainbow) He then used oxygen sensitive bacteria and found them to crowd areas illuminated with red and blue rays of light. This lead to more studies on light and photosynthesis and the role of different coloured compounds called pigments in plants and the utilization of light energy.

Activity-3

Oxygen is produced during Photosynthesis in the presence of light
• Arrange the apparatus as shown in the figure.
• Place some water plant (Elodea or Hydrilla) in a beaker containing pond water and cover these by a shortstemmed
funnel.
• Invert a test-tube full of water over the stem of the funnel. Ensure that the level of water in the beaker is
above the level of stem of the inverted funnel.

Place the apparatus in the sun for at least 3 days (when you leave school keep it back in your classroom). You would see that in place of water there is air that fills in. It is actually a gas that will collect in the test-tube. Test the gas in the test-tube by inserting a glowing incense stick which would burst into flames. This shows the presence of oxygen.

10th Biology - Carbon Dioxide and Photosynthesis - New Syllabus

Carbon dioxide is necessary for Photosynthesis

We need a destarched plant to start with. For destarching we need to keep the plant should in the dark for nearly a week for the removal of starch (or destarching) from the leaves. Arrange the apparatus as shown in the figure.

• Take the wide mouthed transparent bottle.
• Put potassium hydroxide pellets / potassium hydroxide solution in the bottle. (Potassium hydroxide absorbs carbon dioxide)
• Insert splitted cork in the mouth of the bottle.
• Insert one of the leaves of destrached plant (through a split cork) into transparent bottle containing potassium hydroxide dioxide pellets/ potassium hydroxide solution.
• Leave the plant in sunlight.
• After a few hours, test this leaf and any other leaf of this plant for starch.
• The leaf which was exposed to the atmospheric air becomes bluishblack, and the one inside the flask containing potassium hydroxide which absorbs carbon dioxide in the bottle does not become blue-black, showing that carbon dioxide is necessary for photosynthesis.

Ask your teacher why this experiment called Mohl’s of leaf experiment. Write information in your note book.

10th Biology - Water and Photosysthesis - New Syllabus

Water and Photosynthesis

In class VII we had already studied how Von Helmont found that water was essential for the increase of plant mass
He did not know about photosynthesis then. It was later found that increase in plant body mass or material occurred due to the process of photosynthesis. We shall study more about it in the following sections.

Once again read the chapter on nutrition in plants in class VII and write a note on Von Helmont’s experiment focusing on how he concluded that water was important for growth in plant body mass.

Air and Photosynthesis

Let us discuss a simple experiment about Photosynthesis. We have studied some others in our earlier classes. This one helps us to find out about the role of air in the process of photosynthesis. It is interesting to learn about the experiment which was one of the several milestones in the gradual development of our understanding of Photosynthesis.

Joseph Priestly (1733-1804) in 1770 performed a series of experiments that revealed the essential role of air in the growth of green plants (photosynthesis was still not known to scientists). Priestly, you may recall, discovered oxygen in 1774(the name oxygen was coined later by Lavoisier in the year 1775).

Priestly observed that a candle burning in a closed space, a bell jar, soon gets extinguished. Similarly, a mouse would soon suffocate in a closed space of the bell jar. He concluded that a burning candle or an animal, both somehow, damage air. But when he placed a mint plant in the same bell jars, he found that the mouse stayed alive and the candle when lighted from outside continued burning in the presence of the mint plant. Priestly hypothesized as follows: Plants restore to the air whatever breathing animals and burning candles remove.

What had Priestly done to introduce the mint plant without disturbing the experimental set up?
How did he light the candle from outside?
Priestey’s experiment confirms that gaseous exchange was going on and plants were giving out a gas that supported burning and was essential for the survival of animals.

But how do plants takein air and utilize carbon dioxide for photosynthesis and oxygen for respiration?
How do they make the choice?

Massive amounts of gaseous exchange occurs through the stomata present in leaves as long as they are open while plants also carry on gaseous exchange through loose tissues on stems, roots etc. It is actually at the level of the organelles involved in the process of photosynthesis and respiration that the choice of the gas required is made.